![]() Characterization of Self-Assembled Protein Scaffolds from Collagen-mimetic Peptides Devyani Shukla, Jayeeta Lahiri, and Avanish Singh Parmar 19. Genipin Cross-linking Of Elastin and Elastin-based Proteins Lisa D. Cross-linked Collagen Gels using Gold Nanoparticles Katarzyna Slowinska 17. ElSohly, Chawita Netirojjanakul, and Matthew B. Antibody Modification of p-aminophenylalanine-containing Proteins Adel M. Modification of Protein Scaffolds via Copper-catalyzed Azide-Alkyne Cycloaddition Stanislav Presolski 15. Incorporation of Non-canonical Amino Acids into Proteins by Global Reassignment of Sense Codons Katharine Y. Type III Secretion Filaments as Templates for Metallic Nanostructure Synthesis Anum Azam Glasgow 13. Synthesis of Triangular Silver and Gold Nanoprisms Using Consensus Sequence Tetratricopeptide Repeat Proteins Xi Geng and Tijana Z. TMV Disk Scaffolds for Making sub-30 nm Silver Nanorings Serene Bayram, Omar Zahr, Julia Del Re, and Amy Szuchmacher Blum 11. TMV Disk Scaffolds for Making sub-30 nm Silver Nanorings Serene Bayram, Omar Zahr, Julia Del Re, and Amy Szuchmacher Blum 10. Synthesis of Metal-organic Frameworks on Tobacco Mosaic Virus Templates Shaobo Li and Jeremiah J. Synthesis of Cross-linked 2-aminoethyl Methacrylate in P22 Viral Capsid via Atom-transfer Radical Polymerization Shefah Qazi 8. Modification of CCMV Nanocages for Enzyme Encapsulation Lise Schoonen and Jan C. Metal Ion-induced Self-Assembly and Packaging of CCMV Nanocapsules Lise Schoonen, Mark B. Enzyme Encapsulation in an Engineered Lumazine Synthase Protein Cage Yusuke Azuma and Donald Hilvert 5. Encapsulation of Exogenous Proteins in Vault Nanoparticles Meng Wang, Danny Abad, Valerie A. Encapsulation of Active Enzymes within Bacteriophage P22 virus-like Particles Dustin P. Nanoreactors via Encapsulation of Catalytic Gold Nanoparticles within Cowpea Chlorotic Mottle Virus Aijie Liu, Mark V. Authoritative and cutting-edge, Protein Scaffolds: Design, Synthesis, and Applications aims to ensure successful results in the study of this vital field.ฤก. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Site-specific bioconjugation methods, some unique protein architectures, and techniques that exploit peptide amphiphile micelles and assembly of chaperones are also featured. Chapters guide the readers through exploiting a number of protein scaffolds including virus nanoparticles, elastin and collagen peptides and proteins, and other protein templates for either building materials or presentation of ligands. This volume provides a wide range of methods and protocols detailing various protein structures as platforms for building architectures with targeted application.
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